A physical and functional link between splicing factors promotes pre-mRNA 3′ end processing

@inproceedings{Millevoi2009APA,
  title={A physical and functional link between splicing factors promotes pre-mRNA 3′ end processing},
  author={Stefania Millevoi and Adrien Decorsi{\`e}re and Clarisse Loulergue and Jason Iacovoni and Sandra Bernat and Michael N Antoniou and St{\'e}phan Vagner},
  booktitle={Nucleic acids research},
  year={2009}
}
Polypyrimidine tract-binding protein (PTB) is a splicing regulator that also plays a positive role in pre-mRNA 3' end processing when bound upstream of the polyadenylation signal (pA signal). Here, we address the mechanism of PTB stimulatory function in mRNA 3' end formation. We identify PTB as the protein factor whose binding to the human beta-globin (HBB) 3' UTR is abrogated by a 3' end processing-inactivating mutation. We show that PTB promotes both in vitro 3' end cleavage and… CONTINUE READING