A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family.

@article{Smith2000APC,
  title={A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family.},
  author={Jeff Smith and Carrie Baker Brachmann and Ivana Celic and Margaret A. Kenna and Safia Muhammad and Vincent J Starai and Jos{\'e} L Avalos and Jorge C Escalante-Semerena and Charles Grubmeyer and Cynthia Wolberger and Jef D. Boeke},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 12},
  pages={6658-63}
}
The yeast Sir2 protein, required for transcriptional silencing, has an NAD(+)-dependent histone deacetylase (HDA) activity. Yeast extracts contain a NAD(+)-dependent HDA activity that is eliminated in a yeast strain from which SIR2 and its four homologs have been deleted. This HDA activity is also displayed by purified yeast Sir2p and homologous Archaeal, eubacterial, and human proteins, and depends completely on NAD(+) in all species tested. The yeast NPT1 gene, encoding an important NAD… CONTINUE READING
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Methods in Yeast Genetics. A Laboratory Course Manual (Cold

  • M. D. Rose, F. Winston, P. Heiter
  • 1990

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