A photoreversible conformational change in 124 kDa Avena phytochrome.

  title={A photoreversible conformational change in 124 kDa Avena phytochrome.},
  author={Bal Ram Singh and Y G Chai and Pill Soon Song and Jong-Hee Lee and George W. Robinson},
  journal={Biochimica et biophysica acta},
  volume={936 3},
Tryptophan (Trp) fluorescence quenching of phytochrome has been studied using anionic, cationic and neutral quenchers, I-, Cs+ and acrylamide, respectively, in an effort to understand the molecular differences between the Pr and Pfr forms. The data have been analyzed using both Stern-Volmer and modified Stern-Volmer kinetic treatments. The anionic quencher, I-, was proven to be an ineffective quencher with Stern-Volmer constants, Ksv, of 0.60 and 0.63 M-1, respectively, for the Pr and Pfr forms… CONTINUE READING

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