A photolithographic method to create cellular micropatterns.

@article{Karp2006APM,
  title={A photolithographic method to create cellular micropatterns.},
  author={Jeffrey M Karp and Yoon Yeo and W D Geng and Christopher Cannizarro and Kenny Yan and Daniel S. Kohane and Gordana Vunjak-Novakovic and Robert S Langer and Milica Radisic},
  journal={Biomaterials},
  year={2006},
  volume={27 27},
  pages={
          4755-64
        }
}
Here we describe a simple and rapid system for creation of patterned cell culture substrates. This technique is based on (1) printing a mask on a standard overhead transparency, (2) coating a thin layer of a photocrosslinkable chitosan on a slide, (3) exposing the slide and mask to ultraviolet (UV) light, and (4) rinsing the uncrosslinked polymer to expose the underlying cell-repellent patterns. Photocross-linkable chitosan does not require photoinitiators, it is non-toxic and forms flexible… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 29 REFERENCES

Photocrosslinkable chitosan as a biological adhesive.

  • Journal of biomedical materials research
  • 2000
VIEW 5 EXCERPTS
HIGHLY INFLUENTIAL

Medium perfusion enables engineering of compact and contractile cardiac tissue.

  • American journal of physiology. Heart and circulatory physiology
  • 2004
VIEW 2 EXCERPTS