A phosphonate monoester hydrolase from Burkholderia caryophilli PG2982 is useful as a conditional lethal gene in plants.

@article{Dotson1996APM,
  title={A phosphonate monoester hydrolase from Burkholderia caryophilli PG2982 is useful as a conditional lethal gene in plants.},
  author={Stan B Dotson and Michael B. Lanahan and Alan G. Smith and Ganesh M. Kishore},
  journal={The Plant journal : for cell and molecular biology},
  year={1996},
  volume={10 2},
  pages={383-92}
}
A bacterial phosphonate monoester hydrolase was evaluated in plants as a conditional lethal gene useful for cell ablation and negative selection. Glyphosate is a potent herbicide whereas its phosphonate monoester derivative, glyceryl glyphosate, is approximately 50-fold less active. A phosphonate monoesterase gene (pehA) encoding an enzyme that hydrolyzes phosphonate esters including glyceryl glyphosate to glyphosate and glycerol was cloned from the glyphosate metabolizing bacterium… CONTINUE READING