A phosphodiesterase 4 inhibitor, roflumilast N-oxide, inhibits human lung fibroblast functions in vitro.

@article{Sabatini2010AP4,
  title={A phosphodiesterase 4 inhibitor, roflumilast N-oxide, inhibits human lung fibroblast functions in vitro.},
  author={Federica Sabatini and Loredana Petecchia and Silvia Boero and Michela Silvestri and Juergen Klar and Herman Tenor and Rolf Beume and Armin Hatzelmann and Giovanni A Rossi},
  journal={Pulmonary pharmacology & therapeutics},
  year={2010},
  volume={23 4},
  pages={283-91}
}
The PDE4 inhibitor roflumilast mitigates bleomycin-induced lung fibrotic remodeling in rodents. In the current study it was explored whether roflumilast N-oxide, the active metabolite of roflumilast influences functions of cultured lung fibroblasts. Cells of the human foetal lung fibroblast strain GM06114 were stimulated with TNF-alpha (5 ng ml(-1)) and cell surface ICAM-1 and eotaxin release were assessed. [methyl-(3)H] thymidine incorporation was measured following stimulation with bFGF (10… CONTINUE READING