A phorbol ester‐binding protein is required downstream of Rab5 in endosome fusion

@article{Aballay1998APE,
  title={A phorbol ester‐binding protein is required downstream of Rab5 in endosome fusion},
  author={A. Aballay and M. Barbieri and M. Colombo and G. Arenas and P. Stahl and L. Mayorga},
  journal={FEBS Letters},
  year={1998},
  volume={441}
}
Previous observations indicate that a zinc and phorbol ester binding factor is necessary for endosome fusion. To further characterize the role of this factor in the process, we used an in vitro endosome fusion assay supplemented with recombinant Rab5 proteins. Both zinc depletion and addition of calphostin C, an inhibitor of protein kinase C, inhibited endosome fusion in the presence of active Rab5. Addition of the phorbol ester PMA (phorbol 12‐myristate 13‐acetate) reversed the inhibition of… Expand
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