A peptide tag system for facile purification and single-molecule immobilization.

Abstract

A peptide fusion tag and accompanying recombinant capture reagents have been developed on the basis of the peptide-PDZ domain interaction and affinity clamps, a new class of affinity reagent. This system allows for single-step purification under mild conditions and stable capture of a tagged protein. The subnanomolar affinity, high force resistance (>30 pN… (More)
DOI: 10.1021/bi901756n

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@article{Huang2009APT, title={A peptide tag system for facile purification and single-molecule immobilization.}, author={Jin Huang and Stanislav Nagy and Akiko Koide and Ronald S. Rock and Shohei Koide}, journal={Biochemistry}, year={2009}, volume={48 50}, pages={11834-6} }