A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor protease.

@article{Cummings2002APF,
  title={A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor protease.},
  author={Richard T. Cummings and Scott P. Salowe and Barry R. Cunningham and Judyann Wiltsie and Young Whan Park and Lisa M. Sonatore and Douglas Wisniewski and Cameron M. Douglas and Jeffrey D. Hermes and Edward M. Scolnick},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 10},
  pages={
          6603-6
        }
}
A fluorescence resonance energy transfer assay has been developed for monitoring Bacillus anthracis lethal factor (LF) protease activity. A fluorogenic 16-mer peptide based on the known LF protease substrate MEK1 was synthesized and found to be cleaved by the enzyme at the anticipated site. Extension of this work to a fluorogenic 19-mer peptide, derived, in part, from a consensus sequence of known LF protease targets, produced a much better substrate, cleaving approximately 100 times more… CONTINUE READING

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