A pathway for mitotic chromosome formation

@article{Gibcus2018APF,
  title={A pathway for mitotic chromosome formation},
  author={Johan H Gibcus and Kumiko Samejima and Anton Goloborodko and Itaru Samejima and Natalia Naumova and Johannes Nuebler and Masato T Kanemaki and Linfeng Xie and James R Paulson and William C Earnshaw and Leonid A. Mirny and Job Dekker},
  journal={Science},
  year={2018},
  volume={359}
}
Mitotic chromosomes fold as compact arrays of chromatin loops. To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed. During prometaphase, ~80-kb inner loops are nested within ~400-kb outer loops. The loop array acquires a helical… CONTINUE READING
BETA

Citations

Publications citing this paper.
SHOWING 1-10 OF 30 CITATIONS

Condensins and cohesins - one of these things is not like the other!

  • Journal of cell science
  • 2019
VIEW 4 EXCERPTS
CITES BACKGROUND & RESULTS
HIGHLY INFLUENCED

Absolute quantification of cohesin, CTCF and their regulators in human cells

Johann Holzmann, Antonio Z Politi, +14 authors Jan-Michael Peters
  • eLife
  • 2019

References

Publications referenced by this paper.
SHOWING 1-10 OF 95 REFERENCES

Organization of the mitotic chromosome

N. Naumova
  • Science 342,
  • 2013
VIEW 10 EXCERPTS
HIGHLY INFLUENTIAL

An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells

H. Hochegger
  • J. Cell Biol. 178,
  • 2007
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Laemmli, The structure of histone-depleted metaphase chromosomes

U.K.J.R. Paulson
  • Cell 12,
  • 1977
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

Rapid degradation of condensins and 3D-EM reveal chromatin volume is uncoupled from chromosome architecture in mitosis

K. Samejima
  • J. Cell Sci
  • 2018
VIEW 1 EXCERPT

Similar Papers

Loading similar papers…