A pathway‐specific transcriptional regulatory gene for nikkomycin biosynthesis in Streptomyces ansochromogenes that also influences colony development

@article{Liu2005APT,
  title={A pathway‐specific transcriptional regulatory gene for nikkomycin biosynthesis in Streptomyces ansochromogenes that also influences colony development},
  author={Gang Liu and Yuqing Tian and Haihua Yang and H. Tan},
  journal={Molecular Microbiology},
  year={2005},
  volume={55}
}
DNA sequence analysis of a 7.5 kb XhoI DNA fragment from the region flanking the nikkomycin biosynthesis gene cluster in Streptomyces ansochromogenes revealed one 3.3 kb open reading frame (ORF), designated sanG. The deduced product of sanG (1061 amino acids), which is similar to PimR of Streptomyces natalensis, contains an OmpR‐like DNA binding domain in its N‐terminal portion and A‐ and B‐type nucleotide binding motifs in the middle of the protein. Disruption of sanG abolished nikkomycin… Expand
SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes
TLDR
It is indicated that SabR modulated nikkomycin biosynthesis as an enhancer via interaction with the promoter region of sanG, and expanded the understanding about regulatory cascade in nikkomersin biosynthesis. Expand
The pleiotropic regulator AdpA‐L directly controls the pathway‐specific activator of nikkomycin biosynthesis in Streptomyces ansochromogenes
TLDR
Gene disruption and genetic complementation revealed that adpA‐L was required for both nikkomycin biosynthesis and morphological differentiation, highlighting a new level of complexity in the regulation of nikkomersin biosynthesis. Expand
polR, a pathway-specific transcriptional regulatory gene, positively controls polyoxin biosynthesis in Streptomyces cacaoi subsp. asoensis.
  • Rui Li, Z. Xie, +6 authors H. Tan
  • Biology, Medicine
  • Microbiology
  • 2009
TLDR
The data suggest that PolR acts in a positive manner to regulate POL production by activating the transcription of at least two putative operons in the pol gene cluster. Expand
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TLDR
Results indicated that the ATPase/GTPase activity of SanG modulated the transcriptional activation ofSanG target genes during nikkomycin biosynthesis. Expand
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TLDR
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TLDR
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The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997
TLDR
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sanN encoding a dehydrogenase is essential for Nikkomycin biosynthesis in Streptomyces ansochromogenes.
TLDR
Purified recombinant SanN acts as a dehydrogenase to convert benzoate-CoA to benzaldehyde in a random-order mechanism in vitro, with respective Kcat/Km values suggesting that SanN catalyzes the formation of picolinaldehyde during biosynthesis of nikkomycin X and Z components in the wild-type stain. Expand
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References

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Cloning, sequencing and function ofsanB, a gene related to nikkomycin biosynthesis ofStreptomyces ansochromogenes
TLDR
It was found that disruptants ofsanB lost the ability to synthesize nikkomycin, which reveals thatsanB is a novel gene essential for nikkomecin biosynthesis. Expand
The Streptomyces tendae Tü901 L-lysine 2-aminotransferase catalyzes the initial reaction in nikkomycin D biosynthesis.
TLDR
The results identified the nikC-encoded L-lysine 2-aminotransferase as the nikkomycin biosynthetic enzyme that catalyzes the initial reaction in nikomycin D biosynthesis. Expand
Structure and Function of sanV: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes
TLDR
Gene disruption was performed to study the function of sanV and it was found that sanV disruptants exhibited much poorer inhibition to the test strain than the wild-type S. ansochromogenes 7100, which revealed thatsanV is an important gene involved in the biosynthesis of the peptidyl moiety of nikkomycins. Expand
Molecular characterization of co-transcribed genes from Streptomyces tendae Tü901 involved in the biosynthesis of the peptidyl moiety and assembly of the peptidyl nucleoside antibiotic nikkomycin
TLDR
The results indicate that nikU and nikV are required for the synthesis of hydroxypyridylhomothreonine, the genes nikP1, nkP2 and nIKS are necessary for the assembly of nikkomycins, and nkT is required for both pathways. Expand
Characterization of the Pathway-Specific Positive Transcriptional Regulator for Actinorhodin Biosynthesis inStreptomyces coelicolor A3(2) as a DNA-Binding Protein
TLDR
Although both gene products (wild-type and mutant ActII-ORF4) showed DNA-binding activity, only the wild-type gene was capable of activating transcription of the act genes; thus, two basic functions can be differentiated within the regulatory protein. Expand
Mapping the DNA‐binding domain and target sequences of the Streptomyces peucetius daunorubicin biosynthesis regulatory protein, DnrI
TLDR
The spacing between the repeat target sequences is critical for successful occupation by DnrI and, therefore, competent transcriptional activation of the dnrG–dpsABCD operon and the mutant promoter fragments were found to have modified binding characteristics in gel mobility shift assays. Expand
Molecular characterization of co-transcribed genes from Streptomyces tendae Tü901 involved in the biosynthesis of the peptidyl moiety of the peptidyl nucleoside antibiotic nikkomycin
TLDR
Six genes from Streptomyces tendae Tü901 are identified by sequencing the region surrounding the nikC gene, which encodes L-lysine 2-aminotransferase, and it is indicated that they function in the biosynthetic pathway leading to hydroxypyridylhomothreonine. Expand
Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2)
TLDR
The putative redD polypeptide lacked any strong sequence similarities to other known proteins and was not involved in the regulation of undecylprodigiosin (Red) biosynthesis in Streptomyces coelicolor. Expand
Identification of PimR as a Positive Regulator of Pimaricin Biosynthesis in Streptomyces natalensis
TLDR
Gene expression analysis by reverse transcriptase PCR of the pimaricin gene cluster revealed that S. natalensis DeltaPimR shows no expression at all of the cholesterol oxidase-encoding gene pimE, and very low level transcription of the remaining genes of the cluster except for the mutant pimR gene, demonstrating that this regulator activates the transcription of all the genes belonging to the pimAricin genes but not its own transcription. Expand
Cloning, Sequencing, and Function of sanF: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes
TLDR
The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomecin biosynthesis. Expand
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