A pH-dependent conformational change, rather than the chemical step, appears to be rate-limiting in the hammerhead ribozyme cleavage reaction.

@article{Murray2002APC,
  title={A pH-dependent conformational change, rather than the chemical step, appears to be rate-limiting in the hammerhead ribozyme cleavage reaction.},
  author={James B Murray and Christine M. Dunham and William G. Scott},
  journal={Journal of molecular biology},
  year={2002},
  volume={315 2},
  pages={
          121-30
        }
}
  • James B Murray, Christine M. Dunham, William G. Scott
  • Published 2002
  • Medicine, Chemistry
  • Journal of molecular biology
  • We have investigated the chemical basis for a previously observed 7.8 A conformational change in the hammerhead ribozyme that positions the substrate for in-line attack. We have found that the conformational change can only be observed at or above pH 8.5 (in the presence of Co(2+)) and requires the presence of an ionizable 2'-OH at the cleavage site, and note that this observed apparent pK(a) of 8.5 for the conformational change is within experimental error (+/-0.5) of the previously reported… CONTINUE READING

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    The crystal structure of an all-RNA hammerhead ribozyme.

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