A one-tube nested polymerase chain reaction for the detection of mycobacterium bovis in spiked milk samples: an evaluation of concentration and lytic techniques.

@article{Antognoli2001AON,
  title={A one-tube nested polymerase chain reaction for the detection of mycobacterium bovis in spiked milk samples: an evaluation of concentration and lytic techniques.},
  author={Mar{\'i}a C Antognoli and Mo D. Salman and Joni M Triantis and J. Vergara Hern{\'a}ndez and Tom Keefe},
  journal={Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc},
  year={2001},
  volume={13 2},
  pages={111-6}
}
The objective of this study was to evaluate the use of a one-tube nested polymerase chain reaction (OTN PCR) with 5 concentration and lytic treatments for the detection of Mycobacterium bovis in experimentally inoculated milk samples (spiked samples). OTN PCR and the following treatments were tested in inoculated samples: 1) centrifugation; 2) C18-carboxypropylbetaine + capture resin 1 + Proteinase K (CB18-CH-PK); 3) centrifugation + capture resin 1 + Proteinase K; 4) centrifugation + capture… CONTINUE READING

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