A one-step technique for the subcellular fractionation of total cell homogenates.

@article{Morand1986AOT,
  title={A one-step technique for the subcellular fractionation of total cell homogenates.},
  author={J N Morand and Claudia Kent},
  journal={Analytical biochemistry},
  year={1986},
  volume={159 1},
  pages={157-62}
}
A procedure was developed for the rapid, analytical subcellular fractionation of entire homogenates from the Chinese hamster ovary and HeLa cell lines. The procedure avoids a nuclear sedimentation step and the losses that accompany such a step. A key to the development of this procedure was the addition to homogenates of either micrococcal nuclease or DNase I. Nuclease-treated homogenates were fractionated on self-forming Percoll gradients. The entire procedure from cell harvesting through… CONTINUE READING