A one-step SYBR Green I-based product-enhanced reverse transcriptase assay for the quantitation of retroviruses in cell culture supernatants.

@article{Pizzato2009AOS,
  title={A one-step SYBR Green I-based product-enhanced reverse transcriptase assay for the quantitation of retroviruses in cell culture supernatants.},
  author={Massimo Pizzato and Otto Erlwein and David Bonsall and Stephen Kaye and David Muir and Myra O. McClure},
  journal={Journal of virological methods},
  year={2009},
  volume={156 1-2},
  pages={
          1-7
        }
}
  • Massimo Pizzato, Otto Erlwein, +3 authors Myra O. McClure
  • Published 2009
  • Biology, Medicine
  • Journal of virological methods
  • PCR-enhanced reverse transcriptase assays (PERT) are sensitive tools for the detection of retroviruses in biological samples. The adaptation of real-time PCR techniques based on fluorescent probes (F-PERT) has added a reliable quantitative capacity to the assay. In the interest of economy and time, the SYBR Green I-based real-time detection system was used to establish a convenient one-step PERT assay (SG-PERT). This assay can be completed in 2h, is linear over six orders of magnitude and can… CONTINUE READING

    Citations

    Publications citing this paper.
    SHOWING 1-10 OF 104 CITATIONS, ESTIMATED 73% COVERAGE

    FILTER CITATIONS BY YEAR

    2009
    2020

    CITATION STATISTICS

    • 9 Highly Influenced Citations

    • Averaged 13 Citations per year from 2018 through 2020

    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 37 REFERENCES

    A modified single-tube one-step product-enhanced reverse transcriptase (mSTOS-PERT) assay with heparin as DNA polymerase inhibitor for specific detection of RTase activity.