A one-step SYBR Green I-based product-enhanced reverse transcriptase assay for the quantitation of retroviruses in cell culture supernatants.

@article{Pizzato2009AOS,
  title={A one-step SYBR Green I-based product-enhanced reverse transcriptase assay for the quantitation of retroviruses in cell culture supernatants.},
  author={Massimo Pizzato and Otto Erlwein and David Bonsall and Stephen Kaye and David Muir and Myra O. McClure},
  journal={Journal of virological methods},
  year={2009},
  volume={156 1-2},
  pages={1-7}
}
PCR-enhanced reverse transcriptase assays (PERT) are sensitive tools for the detection of retroviruses in biological samples. The adaptation of real-time PCR techniques based on fluorescent probes (F-PERT) has added a reliable quantitative capacity to the assay. In the interest of economy and time, the SYBR Green I-based real-time detection system was used to establish a convenient one-step PERT assay (SG-PERT). This assay can be completed in 2h, is linear over six orders of magnitude and can… CONTINUE READING
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