A one-step DNA PCR-based method for the detection of Mycobacterium tuberculosis complex grown on Lowenstein-Jensen media.

@article{Elbir2008AOD,
  title={A one-step DNA PCR-based method for the detection of Mycobacterium tuberculosis complex grown on Lowenstein-Jensen media.},
  author={Haitham Elbir and Abdel-Muhsin A. Abdel-Muhsin and Ahmed Hussein Babiker},
  journal={The American journal of tropical medicine and hygiene},
  year={2008},
  volume={78 2},
  pages={316-7}
}
A simple, rapid, and sensitive direct colony polymerase chain reaction (PCR) method to detect Mycobacterium tuberculosis complex grown on Lowenstein-Jensen media is described. M. tuberculosis is killed by treating it for 2 hours with 70% ethanol. Whole Mycobacterium cells inactivated by ethanol are added to a PCR mix that is designed to amplify the IS6110 insertion sequence. All 44 isolates tested were positive by this method. Our results show that PCR can be performed directly on bacterial… CONTINUE READING
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