A nutrient‐regulated, dual localization phospholipase A2 in the symbiotic fungus Tuber borchii

@article{Soragni2001AND,
  title={A nutrient‐regulated, dual localization phospholipase A2 in the symbiotic fungus Tuber borchii},
  author={E. Soragni and A. Bolchi and R. Balestrini and C. Gambaretto and R. Percudani and P. Bonfante and S. Ottonello},
  journal={The EMBO Journal},
  year={2001},
  volume={20}
}
Important morphogenetic transitions in fungi are triggered by starvation‐induced changes in the expression of structural surface proteins. Here, we report that nutrient deprivation causes a strong and reversible up‐regulation of TbSP1, a surface‐associated, Ca2+‐dependent phospholipase from the mycorrhizal fungus Tuber borchii. TbSP1 is the first phospholipase A2 to be described in fungi and identifies a novel class of phospholipid‐hydrolyzing enzymes. The TbSP1 phospholipase, which is… Expand
Phospholipase A2 up-regulation during mycorrhiza formation in Tuber borchii.
TLDR
It is shown that TbSP1 appears to respond to two partially overlapping yet distinct stimuli: nutrient starvation and mycorrhiza formation. Expand
Autoproteolytic Activation of a Symbiosis-regulated Truffle Phospholipase A2*
TLDR
Autoproteolytic activation is described for the first time for a microbial PLA2, which may strengthen and further control the effects of phospholipase up-regulation in response to nutrient deprivation, also in the context of symbiosis establishment and mycorrhiza formation. Expand
Functional properties and differential mode of regulation of the nitrate transporter from a plant symbiotic ascomycete.
TLDR
The functional and expression properties, described in the present study, delineate TbNrt2 as a versatile transporter that may be especially suited to cope with the fluctuating (and often low) mineral nitrogen concentrations found in most natural, especially forest, soils. Expand
Distinct enzymatic and cellular characteristics of two secretory phospholipases A2 in the filamentous fungus Aspergillus oryzae.
TLDR
Experiments with sPLA(2) overexpressing strains demonstrated that two enzymes produce subtly different phospholipid composition variations and also differ in their subcellular localization: sPlaA is most abundant in hyphal tips and secreted to the medium, whereas sPlAB predominantly localizes to the ER-like intracellular compartment. Expand
Morphological and Molecular Modifications Induced by Different Carbohydrate Sources in Tuber borchii
TLDR
The increased expression of genes involved in morphological modifications might support the hypothesis that glucose can act as a signal for the fungus to indicate the presence of the plant, and to trigger the complex symbiotic process. Expand
The role of the glyoxylate cycle in the symbiotic fungus Tuber borchii: expression analysis and subcellular localization
TLDR
The high induction of TbICL, TbMLS and TbFBP transcription and the translocation of lipids in fruiting bodies let us hypothesize that glyoxylate cycle and gluconeogenesis are key metabolic pathways in the recycling of existing cell material and the channelling towards the biosynthesis of new cell components during the maturation of fruite bodies. Expand
Molecular and functional characterization of a Rho GDP dissociation inhibitor in the filamentous fungus Tuber borchii
TLDR
T. borchii expresses a functional RhoGDI homolog that appears as an important modulator of cytoskeleton reorganization during polarized apical growth that antecedes symbiosis instauration, and is essential for improvements in the production of mycorrhized plant seedlings used in timberland extension programs and fruit body production. Expand
Phospholipase A2 and phospholipase B activities in fungi.
TLDR
Fungi possess a broad range of hydrolytic enzymes that attack neutral lipids and phospholipids, and the significance these enzymes could have for the fungal cells and their interaction with the host is just beginning to become aware. Expand
Phospholipases play multiple cellular roles including growth, stress tolerance, sexual development, and virulence in fungi.
TLDR
Current knowledge about the cellular roles of different classes of phospholipases in fungi is discussed, which regulate numerous processes such as cytoskeletal dynamics, growth, homeostasis, membrane remodeling, nutrient acquisition, secretion, signal transduction, stress tolerance, sexual development, and virulence in various organisms including fungi. Expand
Phospholipid acylhydrolases trigger membrane degradation during fungal sporogenesis.
TLDR
The role of phospholipid-deacylating activity, which was also detected in fruiting bodies of other basidiomycetes, in the fungal lipid metabolism is elucidated and specific expression in the spore-producing tissue provides evidence for the involvement of PLA(1) in spore formation. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 57 REFERENCES
Cell Wall and Secreted Proteins ofCandida albicans: Identification, Function, and Expression
TLDR
Surface mannoproteins are strong immunogens that trigger and modulate the host immune response during candidiasis and may play a role in morphogenesis. Expand
A novel class of ectomycorrhiza-regulated cell wall polypeptides in Pisolithus tinctorius.
TLDR
RNA blot analysis showed that the steady-state level of PtSRAP32 transcripts exhibited a drastic up-regulation when fungal hyphae form the mantle, suggesting that SRAPs may form part of a cell-cell adhesion system needed for aggregation of hyphAE in ectomycorrhizas. Expand
The glyoxylate cycle is required for fungal virulence
TLDR
Findings in fungi, in conjunction with reports that isocitrate lyase is both upregulated and required for the virulence of Mycobacterium tuberculosis, demonstrate the wide-ranging significance of the glyoxylate cycle in microbial pathogenesis. Expand
Initial signalling processes induced by elicitors of ectomycorrhiza-forming fungi in spruce cells can also be triggered by G-protein-activating mastoparan and protein phosphatase-inhibiting cantharidin
TLDR
Results indicate that in the initial signalling events in spruce cells, essential protein phosphorylations occur either as an (auto) phosphorylation of a membrane-bound receptor kinase prior to the activation of a G-protein or (and) immediately downstream of the activated G- protein in aosphorylation cascade and are the basic requirements for the ion fluxes following downstream. Expand
Differential expression of chitin synthase III and IV mRNAs in ascomata of Tuber borchii Vittad.
TLDR
Examination of the expression of distinct Chs mRNAs during fruitbody development points to the different cellular roles that can be played by distinct chitin synthases in the differentiation of spores of sexual origin or in ascoma enlargement promoted by the growth of vegetative hyphae. Expand
Secretion of Cryparin, a Fungal Hydrophobin
TLDR
The binding of cryparin to the cell wall is different from that of other hydrophobins which are reported to require a hydrophobic-hydrophilic interface for assembly, presumably because of the lectin-like properties unique to this hydrophobin. Expand
Developmental Biology of a Plant-Fungus Symbiosis: the Ectomycorrhiza
TLDR
Mycorrhizal symbioses provide model systems for studying fundamental plant and/or fungal processes, such as signal perception and transduction, control of cell cycle, cellular differentiation and morphogenesis, and are of tremendous importance for global agricultural and forestry productivity. Expand
Cell wall proteins of the ectomycorrhizal basidiomycete Pisolithus tinctorius: identification, function, and expression in symbiosis.
TLDR
This review summarizes the knowledge of CWPs in P. tinctorius and considers parallels with other biotrophic fungi as a possible framework for future work. Expand
Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea.
TLDR
The results suggest that hydrophobins may have a role in the elaboration of infective structures by fungi and may fulfill other functions in fungal phytopathogenesis. Expand
Developmental and light regulation of eas, the structural gene for the rodlet protein of Neurospora.
TLDR
Eas is the first cloned conidiation (con) gene of N. crassa that is associated with a phenotypic alteration and is identical to the known gene eas (easily wettable), which is shown to be abundant in illuminated mycelia and conidiophores but is not detectable or is barely detectable in dark-grown Mycelia. Expand
...
1
2
3
4
5
...