A nuclear gene essential for mitochondrial replication suppresses a defect of mitochondrial transcription in Saccharomyces cerevisiae

@article{Lisowsky1988ANG,
  title={A nuclear gene essential for mitochondrial replication suppresses a defect of mitochondrial transcription in Saccharomyces cerevisiae},
  author={Thomas Lisowsky and Georg Michaelis},
  journal={Molecular and General Genetics MGG},
  year={1988},
  volume={214},
  pages={218-223}
}
A genomic DNA fragment from yeast was isolated by transforming a temperature sensitive pet mutant. This mutant, pet-ts 798, has previously been characterized by its altered mitochondrial transcription apparatus. Subcloning and DNA sequencing of the genomic DNA fragment identified a reading frame responsible for the restoration of the pet-ts phenotype. The… CONTINUE READING