A nuclear budding mechanism in transiently arrested cells generates drug-sensitive and drug-resistant cells.

@article{Mansilla2009ANB,
  title={A nuclear budding mechanism in transiently arrested cells generates drug-sensitive and drug-resistant cells.},
  author={Sylvia Mansilla and M. Bataller and J. Portugal},
  journal={Biochemical pharmacology},
  year={2009},
  volume={78 2},
  pages={
          123-32
        }
}
HCT116 (p53(+/+)) human colon carcinoma cells treated with nanomolar concentrations of doxorubicin underwent transient senescence, synthesized DNA, showed endopolyploidization, increased their size and became multinucleated without a significant increase in mitosis. Nuclei underwent a budding process that involved the release of buds outside the nuclear membrane, and some of the buds seemed to escape from the polyploid cells. A clonogenic assay showed that some cells proliferated following the… Expand
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