A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tubers.

@article{Nie2001ANU,
  title={A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tubers.},
  author={Xianzhou Nie and Rudra P. Singh},
  journal={Journal of virological methods},
  year={2001},
  volume={91 1},
  pages={37-49}
}
A multiplex reverse transcription polymerase chain reaction (m-RT-PCR) was developed for the simultaneous detection of five potato viruses and a viroid. The synthesis of cDNAs used for amplification was primed by hexanucleotides (random primers, RP). An RNA extraction procedure employing DNase I, is routinely used to isolate potato viruses and viroid (Potato virus S, PVS; Potato leafroll virus, PLRV; Potato virus X, PVX; Potato virus A and Y, PVA, PVY; and Potato spindle tuber viroid, PSTVd… CONTINUE READING
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