A novel two-step method for the formation of tissue-engineered cartilage by mature bovine chondrocytes: the alginate-recovered-chondrocyte (ARC) method.

@article{Masuda2003ANT,
  title={A novel two-step method for the formation of tissue-engineered cartilage by mature bovine chondrocytes: the alginate-recovered-chondrocyte (ARC) method.},
  author={Koichi Masuda and Robert L. Sah and Michael J Hejna and Eugene J-M A Thonar},
  journal={Journal of orthopaedic research : official publication of the Orthopaedic Research Society},
  year={2003},
  volume={21 1},
  pages={139-48}
}
Most attempts to tissue-engineer cartilage have involved seeding of cultured cells into a biological or synthetic scaffold. We have developed a novel two-step culture approach that makes possible the in vitro formation of cartilaginous-like tissue by mature adult bovine chondrocytes without the aid of a synthetic matrix. The first step consists of culturing chondrocytes under conditions that maintain their rounded shape and their molecular phenotype as assessed by type II collagen and aggrecan… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 75 extracted citations

Similar Papers

Loading similar papers…