A novel two-step enzymatic synthesis of blastose, a β-d-fructofuranosyl-(2↔6)-d-glucopyranose sucrose analogue.

@article{MirandaMolina2017ANT,
  title={A novel two-step enzymatic synthesis of blastose, a β-d-fructofuranosyl-(2↔6)-d-glucopyranose sucrose analogue.},
  author={Alfonso Miranda-Molina and Edmundo Castillo and Agust{\'i}n L{\'o}pez Mungu{\'i}a},
  journal={Food chemistry},
  year={2017},
  volume={227},
  pages={202-210}
}
Blastose, a natural disaccharide found in honey, is usually found as a byproduct of fructo-oligosaccharide synthesis from sucrose with fructosyltransferases. In this study, we describe a novel two-step biosynthetic route to obtain blastose, designed from a detailed observation of B. subtilis levansucrase (SacB) acceptor structural requirements for fructosylation. The strategy consisted first in the synthesis of the trisaccharide O-β-d-Fruf-(2↔6)-O-α-d-Glcp-(1↔1)-α-d-Glcp, through a… CONTINUE READING

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