A novel transformation system using a bleomycin resistance marker with chemosensitizers for Aspergillus oryzae.

Abstract

Aspergillus oryzae is resistant to many kinds of antibiotics, which hampers their use to select transformants. In fact, the fungus is resistant to over 200microg/ml of bleomycin (Bm). By enhancing the susceptibility of A. oryzae to Bm using Triton X-100 as a detergent and an ATP-binding cassette (ABC) pump inhibitor, chlorpromazine, to the growing medium… (More)
DOI: 10.1016/j.bbrc.2009.03.108

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