A novel toluene-3-monooxygenase pathway cloned from Pseudomonas pickettii PKO1.

@article{Olsen1994ANT,
  title={A novel toluene-3-monooxygenase pathway cloned from Pseudomonas pickettii PKO1.},
  author={Ronald H. Olsen and Jerome J. Kukor and B Kaphammer},
  journal={Journal of bacteriology},
  year={1994},
  volume={176 12},
  pages={3749-56}
}
Plasmid pRO1957, which contains a 26.5-kb fragment from the chromosome of Pseudomonas pickettii PKO1, allows P. aeruginosa PAO1 to grow on toluene or benzene as a sole carbon and energy source. A subclone of pRO1957, designated pRO1966, when present in P. aeruginosa PAO1 grown in lactate-toluene medium, accumulates m-cresol in the medium, indicating that m-cresol is an intermediate of toluene catabolism. Moreover, incubation of such cells in the presence of 18O2 followed by gas chromatography… CONTINUE READING