A novel strategy to modify adenovirus tropism and enhance transgene delivery to activated vascular endothelial cells in vitro and in vivo.

  title={A novel strategy to modify adenovirus tropism and enhance transgene delivery to activated vascular endothelial cells in vitro and in vivo.},
  author={Ken-ichi Ogawara and M. Rots and R. J. Kok and H. Moorlag and Anne-Miek van Loenen and D. Meijer and H. Haisma and G. Molema},
  journal={Human gene therapy},
  volume={15 5},
  • Ken-ichi Ogawara, M. Rots, +5 authors G. Molema
  • Published 2004
  • Biology, Medicine
  • Human gene therapy
  • To assess the possibilities of retargeting adenovirus to activated endothelial cells, we conjugated bifunctional polyethylene glycol (PEG) onto the adenoviral capsid to inhibit the interaction between viral knob and coxsackie-adenovirus receptor (CAR). Subsequently, we introduced an alphav integrin-specific RGD peptide or E-selectin-specific antibody to the other functional group of the PEG molecule for the retargeting of the adenovirus to activated endothelial cells. In vitro studies showed… CONTINUE READING
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