A novel strategy for the generation of angiostatic kringle regions from a precursor derived from plasminogen

@article{Schmitz2002ANS,
  title={A novel strategy for the generation of angiostatic kringle regions from a precursor derived from plasminogen},
  author={Volker Schmitz and Lin Wang and Miguel A. Barajas and D Peng and Jes{\'u}s Prieto and Cheng Qian},
  journal={Gene Therapy},
  year={2002},
  volume={9},
  pages={1600-1606}
}
In this study we have explored the feasibility of generating angiostatin by incorporating an endoproteolytic furin cleavage site into plasminogen to allow conversion of the precursor molecule into an angiostatic active K1-3 fragment. We show that secretable angiostatin can be successfully generated from cells infected with adenovirus carrying the furin-mutated plasminogen (AdmuthPlgK3). Supernatant from cells transduced with AdmuthPlagK3 inhibits tube formation and proliferation and migration… CONTINUE READING