A novel strategy for rapid and efficient isolation of human tumor-specific CD4(+) and CD8(+) T-cell clones.

@article{Lee2008ANS,
  title={A novel strategy for rapid and efficient isolation of human tumor-specific CD4(+) and CD8(+) T-cell clones.},
  author={Seung-Tae Lee and Shujuan Liu and Laszlo G. Radvanyi and Pariya Sukhumalchandra and Jeffrey J. Molldrem and Eric D. Wieder and Patrick Hwu and Yong-Jun Liu and Larry W. Kwak and Gregory Liz{\'e}e and Sattva S. Neelapu},
  journal={Journal of immunological methods},
  year={2008},
  volume={331 1-2},
  pages={13-26}
}
Adoptive therapy with antigen-specific T cells is a promising approach for the treatment of infectious diseases and cancer. However, cloning of antigen-specific T cells by the traditional approach of limiting dilution is a time-consuming, laborious, and inefficient process. Here, we describe a novel flow cytometric strategy for rapid isolation of human tumor antigen-specific T-cell clones by using T-cell receptor (TCR) Vbeta antibodies in combination with carboxyfluorescein succinimidyl ester… CONTINUE READING