A novel strategy for creating recombinant infectious RNA virus genomes.

  title={A novel strategy for creating recombinant infectious RNA virus genomes.},
  author={Isabelle Fernandez-Delmond and Olivier Pierrugues and M{\'e}lissanne de Wispelaere and Laurent Guilbaud and St{\'e}phane Gaubert and Zolt{\'a}n Div{\'e}ki and Christian Godon and Mark Tepfer and Mireille Jacquemond},
  journal={Journal of virological methods},
  volume={121 2},
Reverse transcriptases with RNase H activity are particularly apt to switch templates and generate recombinant molecules in vitro. This property has been exploited for the first time to create a library of recombinant RNAs 3 between two strains of Cucumber mosaic virus (CMV) or between CMV and Tomato aspermy virus (TAV), which share 75 and 63% sequence identity, respectively. The recombination events were almost entirely of the precise homologous type, and occurred at the same sites as those… CONTINUE READING
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