A novel strategy for creating recombinant infectious RNA virus genomes.

@article{FernandezDelmond2004ANS,
  title={A novel strategy for creating recombinant infectious RNA virus genomes.},
  author={Isabelle Fernandez-Delmond and Olivier Pierrugues and M{\'e}lissanne de Wispelaere and Laurent Guilbaud and St{\'e}phane Gaubert and Zolt{\'a}n Div{\'e}ki and Christian Godon and Mark Tepfer and Mireille Jacquemond},
  journal={Journal of virological methods},
  year={2004},
  volume={121 2},
  pages={
          247-57
        }
}
Reverse transcriptases with RNase H activity are particularly apt to switch templates and generate recombinant molecules in vitro. This property has been exploited for the first time to create a library of recombinant RNAs 3 between two strains of Cucumber mosaic virus (CMV) or between CMV and Tomato aspermy virus (TAV), which share 75 and 63% sequence identity, respectively. The recombination events were almost entirely of the precise homologous type, and occurred at the same sites as those… CONTINUE READING
Highly Cited
This paper has 20 citations. REVIEW CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations