A novel single molecule analysis of spontaneous and radiation-induced mutation at a mouse tandem repeat locus.

@article{Yauk2002ANS,
  title={A novel single molecule analysis of spontaneous and radiation-induced mutation at a mouse tandem repeat locus.},
  author={Carole Yauk and Yuri E. Dubrova and Gemma R Grant and Alec John Jeffreys},
  journal={Mutation research},
  year={2002},
  volume={500 1-2},
  pages={147-56}
}
Expanded simple tandem repeat (ESTR) loci include some of the most unstable DNA in the mouse genome and have been extensively used in pedigree studies of germline mutation. We now show that repeat DNA instability at the mouse ESTR locus Ms6-hm can also be monitored by single molecule PCR analysis of genomic DNA. Unlike unstable human minisatellites which mutate almost exclusively in the germline by a meiotic recombination-based process, mouse Ms6-hm shows repeat instability both in germinal… CONTINUE READING