A novel serine metallokeratinase from a newly isolated Bacillus pumilus A1 grown on chicken feather meal: biochemical and molecular characterization.

@article{FakhfakhZouari2010ANS,
  title={A novel serine metallokeratinase from a newly isolated Bacillus pumilus A1 grown on chicken feather meal: biochemical and molecular characterization.},
  author={Nahed Fakhfakh-Zouari and Noomen Hmidet and Anissa Haddar and Safia Kanoun and Moncef Nasri},
  journal={Applied biochemistry and biotechnology},
  year={2010},
  volume={162 2},
  pages={329-44}
}
A keratinolytic enzyme (KerA1) secreted by a newly isolated Bacillus pumilus strain A1 cultivated in medium containing chicken feather meal was purified and characterized, and the gene was isolated and sequenced. The molecular mass of the purified enzyme was estimated to be 34,000 Da by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and gel filtration. The optimum pH and temperature for the purified keratinase were 9.0 and 60 degrees C, respectively, using keratin as a… CONTINUE READING