A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2).

@article{Brekasis2003ANS,
  title={A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2).},
  author={Dimitris Brekasis and Mark S. B. Paget},
  journal={The EMBO journal},
  year={2003},
  volume={22 18},
  pages={4856-65}
}
We describe the identification of Rex, a novel redox-sensing repressor that appears to be widespread among Gram-positive bacteria. In Streptomyces coelicolor Rex binds to operator (ROP) sites located upstream of several respiratory genes, including the cydABCD and rex-hemACD operons. The DNA-binding activity of Rex appears to be controlled by the redox poise of the NADH/NAD+ pool. Using electromobility shift and surface plasmon resonance assays we show that NADH, but not NAD+, inhibits the DNA… CONTINUE READING
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