A novel screening system for secretion of heterologous proteins in Bacillus subtilis


High-level production of secretory proteins in Bacillus subtilis leads to a stress response involving the two-component system CssRS and its target genes htrA and htrB. Here, we used this sensing system in a reporter strain in which gfp is under control of P(htrA) , the secretion stress responsive promoter of htrA. Overexpression of heterologous secretory… (More)
DOI: 10.1111/j.1751-7915.2011.00270.x


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