A novel role for ciliary function in atopy: ADGRV1 and DNAH5 interactions.

Abstract

BACKGROUND Atopy, an endotype underlying allergic diseases, has a substantial genetic component. OBJECTIVE Our goal was to identify novel genes associated with atopy in asthma-ascertained families. METHODS We implemented a 3-step analysis strategy in 3 data sets: the Epidemiological Study on the Genetics and Environment of Asthma (EGEA) data set (1660 subjects), the Saguenay-Lac-Saint-Jean study data set (1138 subjects), and the Medical Research Council (MRC) data set (446 subjects). This strategy included a single nucleotide polymorphism (SNP) genome-wide association study (GWAS), the selection of related gene pairs based on statistical filtering of GWAS results, and text-mining filtering using Gene Relationships Across Implicated Loci and SNP-SNP interaction analysis of selected gene pairs. RESULTS We identified the 5q14 locus, harboring the adhesion G protein-coupled receptor V1 (ADGRV1) gene, which showed genome-wide significant association with atopy (rs4916831, meta-analysis P value = 6.8 × 10-9). Statistical filtering of GWAS results followed by text-mining filtering revealed relationships between ADGRV1 and 3 genes showing suggestive association with atopy (P ≤ 10-4). SNP-SNP interaction analysis between ADGRV1 and these 3 genes showed significant interaction between ADGRV1 rs17554723 and 2 correlated SNPs (rs2134256 and rs1354187) within the dynein axonemal heavy chain 5 (DNAH5) gene (Pmeta-int = 3.6 × 10-5 and 6.1 × 10-5, which met the multiple-testing corrected threshold of 7.3 × 10-5). Further conditional analysis indicated that rs2134256 alone accounted for the interaction signal with rs17554723. CONCLUSION Because both DNAH5 and ADGRV1 contribute to ciliary function, this study suggests that ciliary dysfunction might represent a novel mechanism underlying atopy. Combining GWAS and epistasis analysis driven by statistical and knowledge-based evidence represents a promising approach for identifying new genes involved in complex traits.

DOI: 10.1016/j.jaci.2017.06.050

Cite this paper

@article{Sugier2017ANR, title={A novel role for ciliary function in atopy: ADGRV1 and DNAH5 interactions.}, author={P E Sugier and Myriam Brossard and Chlo{\'e} Sarnowski and Amaury Vaysse and Andreanne Morin and Lucile Pain and Patricia Margaritte-Jeannin and M H Dizier and William O. C. M. Cookson and Mark Lathrop and Miriam F Moffatt and Catherine Laprise and Florence Demenais and Emmanuelle Bouzigon}, journal={The Journal of allergy and clinical immunology}, year={2017} }