A novel retron that produces RNA-less msDNA in Escherichia coli using reverse transcriptase.

@article{Lima1997ANR,
  title={A novel retron that produces RNA-less msDNA in Escherichia coli using reverse transcriptase.},
  author={Teresa Maneca Lima and David C. L. Lim},
  journal={Plasmid},
  year={1997},
  volume={38 1},
  pages={
          25-33
        }
}
Bacterial retroelements, or retrons, use reverse transcriptase (RT) to produce a multicopy single-stranded DNA (msDNA) molecule that is covalently linked to RNA. In these studies we show that a retron from Escherichia coli 110, a clinical isolate, produces a novel RNA-less msDNA with a 5' phosphate residue. The msDNA is a 74-nucleotide single-stranded DNA molecule with a stable stem-loop structure without a mismatched base pair. Only the genes encoding msDNA (msd), msdRNA (msr), and RT (ret… CONTINUE READING

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