Transcript profiling of the cone-only Nrl-knockout retina using custom cDNA- microarrays: identification of novel targets of Nrl and of signaling pathways involved in photoreceptor function by
PURPOSE To reconstruct the transcriptional profile of the human adult retina and the genomic map of the genes expressed in this tissue. METHODS Original software was used for the retrieval and analysis of records from UniGene (http://www.ncbi.nlm.nih. gov/UniGene/) pertaining to selected cDNA libraries from adult human retina. RESULTS The 4974 genes reported so far to be expressed in retina were included in a catalog available on the Internet. For each entry, an estimation of the level of expression of the corresponding gene in the retina was provided. A high-resolution genomic map of the human retina was built up by inclusion of 3152 genes showing a precise and unique map assignment. The correspondence was established between 53 gene-orphan retinal diseases and clusters of genes expressed in the retina. CONCLUSIONS The in silico reconstruction of the transcriptional profile of the adult human retina provides preliminary information on the pattern of genomic expression in this tissue. The chromosomal location of many retinal genes, combined with their expression data, should speed up the identification of genes involved in retinal diseases.