A novel recombinant dual human SCF expressed in and purified from silkworm, Bombyx mori, possesses higher bioactivity than recombinant monomeric human SCF.

@article{Han2004ANR,
  title={A novel recombinant dual human SCF expressed in and purified from silkworm, Bombyx mori, possesses higher bioactivity than recombinant monomeric human SCF.},
  author={Junhai Han and Yuhui Zang and Haiqin Lu and Jie Zhu and Junchuan Qin},
  journal={European journal of haematology},
  year={2004},
  volume={72 4},
  pages={273-9}
}
A novel recombinant dual human stem cell factor (rdhSCF) gene was constructed which consisted of a full-length hSCF cDNA plus a truncated hSCF cDNA (1-145 aa), linked by a peptide (GGGGSGGGGSGG) coding region. The rdhSCF gene was cloned into baculovirus transfer vector pAcSecG2T under the polyhedrin promoter control. Silkworm larvae infected with the recombinant virus expressed rdhSCF up to 15,800 units/mL in haemolymph. The specific activity of rdhSCF purified from the haemolymph was up to 3.0… CONTINUE READING