A novel proteomics sample preparation method for secretome analysis of Hypocrea jecorina growing on insoluble substrates.

Analysis of the secretomes of filamentous fungi growing on insoluble lignocellulosic substrates is of major current interest because of the industrial potential of secreted fungal enzymes. Importantly, such studies can help identifying key enzymes from a large arsenal of bioinformatically detected candidates in fungal genomes. We describe a simple, plate… CONTINUE READING