A novel procedure for absolute real-time quantification of gene expression patterns

@inproceedings{Lu2011ANP,
  title={A novel procedure for absolute real-time quantification of gene expression patterns},
  author={Yingqing Lu and Lulu Xie and Jiani Chen},
  booktitle={Plant Methods},
  year={2011}
}
Temporal and tissue-specific patterns of gene expression play important roles in functionality of a biological system. Real-time quantitative polymerase chain reaction (qPCR) technique has been widely applied to single gene expressions, but its potential has not been fully released as most results have been obtained as fold changes relative to control conditions. Absolute quantification of transcripts as an alternative method has yet to gain popularity because of unresolved issues. We propose a… CONTINUE READING
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