A novel prefractionation method combining protein and peptide isoelectric focusing in immobilized pH gradient strips.

@article{Pernemalm2013ANP,
  title={A novel prefractionation method combining protein and peptide isoelectric focusing in immobilized pH gradient strips.},
  author={Maria Pernemalm and Janne Lehti{\"o}},
  journal={Journal of proteome research},
  year={2013},
  volume={12 2},
  pages={1014-9}
}
To increase sensitivity and analytical depth in shotgun proteomics, prefractionation of complex samples is often used. Here we describe a novel prefractionation method, Sandwich high resolution isoelectric focusing, which combines both protein and peptide isoelectric focusing. In the first step, intact proteins are separated on the basis of isoelectric… CONTINUE READING

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