A novel plasmid vector that uses the glucose kinase gene (glkA) for the positive selection of stable gene disruptants in Streptomyces.

@article{Wezel1996ANP,
  title={A novel plasmid vector that uses the glucose kinase gene (glkA) for the positive selection of stable gene disruptants in Streptomyces.},
  author={Gilles P van Wezel and Mervyn Bibb},
  journal={Gene},
  year={1996},
  volume={182 1-2},
  pages={229-30}
}
We describe an Escherichia coli plasmid, pIJ2581, that can be used for the efficient construction of stable gene disruptants and of gene deletions in Streptomyces. Integration of pIJ2581 derivatives carrying chromosomal sequences is achieved by selecting for plasmid-encoded thiostrepton resistance, while plasmid excision is secured by counter-selection of the pIJ2581 glkA gene, which confers sensitivity to 2-deoxyglucose. 

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

Similar Papers

Loading similar papers…