A novel phosphoramidite method for automated synthesis of oligonucleotides on glass supports for biosensor development.

Abstract

Two protocols for functionalization of glass supports with hexaethylene glycol (HEG)-linked oligonucleotides were developed. The first method (standard amidite protocol) made use of the 2-cyanoethyl-phosphoramidite derivative of 4,4'-dimethoxytrityl-protected HEG. This was first coupled to the support by standard solid-phase phosphoramidite chemistry… (More)

Topics

Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.