A novel pathway for the production of H2 S by DAO in rat jejunum.

  title={A novel pathway for the production of H2 S by DAO in rat jejunum.},
  author={S H Tang and Dongdong Huang and N An and Dong Chen and D Zhao},
  journal={Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society},
  volume={28 5},
BACKGROUND Hydrogen sulfide (H2 S) is endogenously generated from L-cysteine (L-Cys) by the enzymes cystathionine-β-synthase (CBS) and cystathionine-γ-Lyase (CSE). Hydrogen sulfide is also produced from D-cysteine (D-Cys) by D-Amino acid oxidase (DAO). METHODS The H2 S production was measured by the methylene blue assay. The expression of DAO was investigated by Western blotting and immunohistochemistry. The short-circuit current (Isc) was recorded using the Ussing chamber technique. KEY… CONTINUE READING
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