A novel pairing process promoted by Escherichia coli RecA protein: inverse DNA and RNA strand exchange.

Abstract

Traditionally, recombination reactions promoted by RecA-like proteins initiate by forming a nucleoprotein filament on a single-stranded DNA (ssDNA), which then pairs with homologous double-stranded DNA (dsDNA). In this paper, we describe a novel pairing process that occurs in an unconventional manner: RecA protein polymerizes along dsDNA to form an active nucleoprotein filament that can pair and exchange strands with homologous ssDNA. Our results demonstrate that this "inverse" reaction is a unique, highly efficient DNA strand exchange reaction that is not due to redistribution of RecA protein from dsDNA to the homologous ssDNA partner. Finally, we demonstrate that the RecA protein-dsDNA filament can also pair and promote strand exchange with ssRNA. This inverse RNA strand exchange reaction is likely responsible for R-loop formation that is required for recombination-dependent DNA replication.

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@article{Zaitsev2000ANP, title={A novel pairing process promoted by Escherichia coli RecA protein: inverse DNA and RNA strand exchange.}, author={Eugene N Zaitsev and Stephen C Kowalczykowski}, journal={Genes & development}, year={2000}, volume={14 6}, pages={740-9} }