A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements.

@article{Franois2004ANM,
  title={A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements.},
  author={Patrice François and Gesu{\`e}le Renzi and Didier Pittet and Manuela Teixeira Bento and Daniel Lew and Stephan Harbarth and Pierre Vaudaux and Jacques Schrenzel},
  journal={Journal of clinical microbiology},
  year={2004},
  volume={42 7},
  pages={3309-12}
}
We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular… CONTINUE READING

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