A novel monoclonal antibody permitting recognition of NKT cells in various mouse strains.

@article{Maruoka1998ANM,
  title={A novel monoclonal antibody permitting recognition of NKT cells in various mouse strains.},
  author={H Maruoka and Yoshinori Ikarashi and Katsuaki Shinohara and Masaaki Miyata and Takashi Sugimura and Masaki Terada and Hiro Wakasugi},
  journal={Biochemical and biophysical research communications},
  year={1998},
  volume={242 2},
  pages={413-8}
}
By immunizing mouse lymphoma cell line tMK-2U derived from intermediate TCR cells of BALB/c nude mouse, U5A2-13 monoclonal antibody (mAb, a rat IgG2a) was established. U5A2-13 antigen (Ag) was expressed on around 65% of TCRint cells in the liver of the various mouse strains including both NK1.1- and NK1.1+ mouse strains, while NK1.1 Ag was expressed only in NK1.1+ C57BL/6 mouse strain. Among CD3+ cells, 26.3% cells co-expressed U5A2-13 Ag and NK1.1+ Ag, while small proportions of the CD3+ cells… CONTINUE READING