A novel mode of nuclease action is revealed by the bacterial Mre11/Rad50 complex

@inproceedings{Lim2015ANM,
  title={A novel mode of nuclease action is revealed by the bacterial Mre11/Rad50 complex},
  author={Chew Theng Lim and Pey Jiun Lai and David R F Leach and Hisaji Maki and Asako Furukohri},
  booktitle={Nucleic acids research},
  year={2015}
}
The Mre11/Rad50 complex is a central player in various genome maintenance pathways. Here, we report a novel mode of nuclease action found for the Escherichia coli Mre11/Rad50 complex, SbcC2/D2 complex (SbcCD). SbcCD cuts off the top of a cruciform DNA by making incisions on both strands and continues cleaving the dsDNA stem at ∼10-bp intervals. Using linear-shaped DNA substrates, we observed that SbcCD cleaved dsDNA using this activity when the substrate was 110 bp long, but that on shorter… CONTINUE READING