A novel minimal-size vector (MIDGE) improves transgene expression in colon carcinoma cells and avoids transfection of undesired DNA.

@article{Schakowski2001ANM,
  title={A novel minimal-size vector (MIDGE) improves transgene expression in colon carcinoma cells and avoids transfection of undesired DNA.},
  author={Frank Schakowski and Marcus Gorschlueter and Claas Junghans and Matthias Schroff and Peter Buttgereit and Carsten G Ziske and Bjoern Schoettker and Sven A. K{\"o}nig-Merediz and Tilman Sauerbruch and Burghardt Wittig and Ingo G. H. Schmidt-Wolf},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2001},
  volume={3 5 Pt 1},
  pages={793-800}
}
Viral and plasmid vectors may cause unwanted immunological side effects resulting from the expression of nontherapeutic genes contained in their sequence. Furthermore, replication-defective viral vectors carry the potential risk of recombination with wild-type viruses or activation of oncogenes. A new vector type for minimalistic, immunologically defined gene expression (MIDGE) may overcome these problems. MIDGE is a minimal-size gene transfer unit containing the expression cassette, including… CONTINUE READING