A novel method using fluorescence microscopy for real-time assessment of ATP release from individual cells.

@article{Corriden2007ANM,
  title={A novel method using fluorescence microscopy for real-time assessment of ATP release from individual cells.},
  author={Ross Corriden and Paul A. Insel and Wolfgang G. Junger},
  journal={American journal of physiology. Cell physiology},
  year={2007},
  volume={293 4},
  pages={C1420-5}
}
Many cell types release ATP in response to mechanical or biochemical stimulation. The mechanisms responsible for this release, however, are not well understood and may differ among different cell types. In addition, there are numerous difficulties associated with studying the dynamics of ATP release immediately outside the cell membrane. Here, we report a new method that allows the visualization and quantification of ATP release by fluorescence microscopy. Our method utilizes a two-enzyme… CONTINUE READING
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