A novel method of cryopreservation without a cryoprotectant for immature somatic embryos of conifer

Abstract

Cryopreservation of embryogenic tissue is an essential storage step in genotype selection and seedling production through somatic embryogenesis. To date, immature conifer somatic embryos, at the proliferation step, were only able to tolerate ultra low temperature after prior cryoprotectant treatments. We report a novel cryopreservation method for conifer… (More)
DOI: 10.1007/s11240-010-9899-x

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@article{Kong2010ANM, title={A novel method of cryopreservation without a cryoprotectant for immature somatic embryos of conifer}, author={L. Kong and Patrick von Aderkas}, journal={Plant Cell, Tissue and Organ Culture (PCTOC)}, year={2010}, volume={106}, pages={115-125} }