A novel method of DNA transfection by laser microbeam cell surgery

  title={A novel method of DNA transfection by laser microbeam cell surgery},
  author={M. Tsukakoshi and S. Kurata and Y. Nomiya and Y. Ikawa and T. Kasuya},
  journal={Applied Physics B},
  • M. Tsukakoshi, S. Kurata, +2 authors T. Kasuya
  • Published 1984
  • Physics
  • Applied Physics B
  • A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Nd:YAG laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions. It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. The method offers a clear… CONTINUE READING
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